Fig. 5From: A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virusSpecificity of purified biNb2. The specificity of purified biNb2 was tested by indirect ELISA in 96-well plates coated with different antigens as against the PEDV S, TGEV N, PRRSV N, PCV-Cap and PRV-gE proteins (100 ng/well). Serially diluted biNb2 in MPBS was added to the wells. Then, the optimal concentration of HRP-conjugated streptavidin was added. Each result is represented as an average ± standard deviation of three replicatesBack to article page