Fig. 3
Photophysical and photochemical characterizations of PIC, PIC–Nal, and PIC–Nal–IRI. a Absorbance spectra of BPD, PIC, and PIC–Nal in DMSO showing overlapping main peaks centered at 435 nm (Soret band) and 690 nm (Q band; wavelength for light activation). b Absorbance spectra of irinotecan (IRI), Nal–IRI, and PIC–Nal–IRI in DMSO. c A comparison of the 690 nm absorbance value of BPD, PIC, PIC–Nal, and PIC–Nal–IRI in DMSO and PBS at a fixed BPD concentration. d Photoactivity of BPD, PIC, PIC–Nal, and PIC–Nal–IRI. Photoactivity is defined in the Methods section. e SOSG reports 1O2 production from free BPD, PIC, PIC–Nal, Nal, and ‘PIC + Nal’ in PBS with and without light activation at 690 nm. (n > 3; **P < 0.01, ***P < 0.001; n.s.: not significant; one-way ANOVA, Tukey’s posthoc test)