Fig. 2

The cellular tropism of THLG-EXO in vitro. a Schematics of DNA constructs used for the production of fusion proteins Her2-mCherry (upper panel); flow cytometric analyses of Her2 from SGC-7901 WT cells and Her2-mcherry-SGC-7901 cells with a FITC labeled anti-Her2 monoclonal antibody (lower panel). b Confocal microscopy images of cellular uptake of THLG-EXO or LG-EXO after 3 h incubation with SGC-7901 WT cells and Her2-mcherry-SGC-7901 cells co-culture model. red shows Her2-mcherry-SGC-7901 cells. Green represents THLG-EXO or LG-EXO. Right panel shows enlarged graphs with frame indicating internalized exosomes in cells (×400). c Flow cytometry analysis of co-culture cells after incubation with THLG-EXO or LG-EXO (upper panel); d quantification of exosomes internalization based on flow cytometry analysis (lower panel). Data are expressed as mean ± SD. n = 5; **p < 0.01