Fig. 5
Antitumor activity of THLG-EXO/5-FU/miR-21i in nude mice xenograft using HCT-1165FR cells. a Representative bioluminescent images of tumor growth in nude mice treated with THLG-EXO, THLG-EXO/miR-21i, THLG-EXO/5-FU or THLG-EXO/5-FU/miR-21i at different time point. Signals were adjusted to the same color scale for the entire time course. b The mean bioluminescence intensity (BLI) for each mouse/group, line chart represented quantification of the relative bioluminescent intensity in tumor site. c Tumor weights of different treated groups. T1, T2, T3 and T4 represented the treatment of THLG-EXO, THLG-EXO/miR-21i, THLG-EXO/5-FU, THLG-EXO/5-FU/miR-21i, respectively. d TUNEL assessments of tumor tissues treated with THLG-EXO, THLG-EXO/miR-21i, THLG-EXO/5-FU, THLG-EXO/5-FU/miR-21i, respectively. e Western blot of hMSH2 and PTEN in the tissue of tumor region were performed for 24 h after administration. Data were expressed as means ± SD. n = 6; *P < 0.05, **P < 0.01 versus the THLG-EXO groups or THLG-EXO/miR-21i group, and #P < 0.05 versus the THLG-EXO/5-FU groups