Fig. 5From: Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR–Cas9 plasmidBacterial S. pyogenes Cas9 protein is successfully translated inside murine macrophages. After 24 h incubation with CRISPR+ Lipofectamine (lanes 1 and 2), CRISPR plasmid only (lanes 3 and 4), PBS only (lane 5), CRISPR loaded nanoparticle (lane 6), and blank nanoparticle (lane 7), S. pyogenes Cas9 protein was detectable by Western BlotBack to article page