Table 1 Size and zeta potential of NPs in DI water made with different end-capped PLGA before and after lyophilization
From: Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR–Cas9 plasmid
Sample | PLGA end cap | Target DNA loading (wt% based on PLGA) | Actual DNA loading (wt% based on PLGA) | Before freeze drying | Freeze dried 1:~ 25 NP:trehalose (w:w) | ||||
|---|---|---|---|---|---|---|---|---|---|
DI (nm) | PDI | ZP (mV) | DI (nm) | PDI | ZP (mV) | ||||
A | Ester (neutral) | – | – | 157 | 0.06 | − 31 | 344 | 0.42 | − 29 |
B | 2 | 0.7 | 160 | 0.11 | − 33 | 213 | 0.23 | − 35 | |
C | Amine (+) | – | – | 177 | 0.11 | − 24 | 290 | 0.37 | − 27 |
D | 2 | 1.6 | 182 | 0.08 | − 26 | 260 | 0.21 | − 30 | |