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Table 1 Size and zeta potential of NPs in DI water made with different end-capped PLGA before and after lyophilization

From: Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR–Cas9 plasmid

SamplePLGA end capTarget DNA loading (wt% based on PLGA)Actual DNA loading (wt% based on PLGA)Before freeze dryingFreeze dried 1:~ 25 NP:trehalose (w:w)
DI (nm)PDIZP (mV)DI (nm)PDIZP (mV)
AEster (neutral)1570.06− 313440.42− 29
B20.71600.11− 332130.23− 35
CAmine (+)1770.11− 242900.37− 27
D21.61820.08− 262600.21− 30