Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

Table 2 Determination of the best pairs of nanobodies, as the capture antibody, and nanobody-HRP fusions as the detection antibody for the sandwich ELISA-like immunoassay to detect PPV by orthogonal assay

Different nanobody-HRP fusions as detection antibody	Samples	Different nanobodies expressed by E. coli as capture antibody
Different nanobody-HRP fusions as detection antibody	Samples	PPV-VP2-Nb5	PPV-VP2-Nb12	PPV-VP2-Nb18	PPV-VP2-Nb19	PPV-VP2-Nb56
PPV-VP2-Nb5-HRP	Positive	0.83	0.60	0.42	0.52	0.49
	Negative	0.19	0.21	0.20	0.21	0.22
	P/N	4.35	2.87	2.10	2.49	2.20
PPV-VP2-Nb12-HRP	Positive	0.78	0.36	0.28	0.29	0.24
	Negative	0.22	0.19	0.21	0.24	0.19
	P/N	3.49	1.88	1.30	1.21	1.26
PPV-VP2-Nb18-HRP	Positive	0.64	0.30	0.23	0.25	0.35
	Negative	0.23	0.21	0.19	0.20	0.21
	P/N	2.80	1.45	1.24	1.25	1.62
PPV-VP2-Nb19-HRP	Positive	0.67	0.35	0.26	0.38	0.30
	Negative	0.19	0.24	0.23	0.25	0.18
	P/N	3.49	1.48	1.12	1.50	1.67
PPV-VP2-Nb56-HRP	Positive	1.23	0.77	0.51	1.50	0.64
	Negative	0.21	0.22	0.21	0.19	0.23
	P/N	5.86	3.52	2.45	7.89	2.79

ISSN: 1477-3155