Table 5 Comparisons between the developed sandwich ELISA-like assay, direct immunofluorescent assay, real-time PCR and commercial monoclonal antibody-based sandwich ELISA by detecting the sequential fecal and serum samples from pigs infected with PPV
Different assays for detecting PPV | Samples from the different dpi of 3 challenged pigs (Number positive of faeces/number positive of sera) | ||||||
|---|---|---|---|---|---|---|---|
3 | 5 | 7 | 10 | 14 | 21 | 28 | |
Developed sandwich ELISA-like assay | 0/0 | 0/0 | 0/2 | 3/3 | 1/0 | 0/0 | 0/0 |
Direct immunofluorescent assay | 0/0 | 0/0 | 0/1 | 2/2 | 0/0 | 0/0 | 0/0 |
Real-time PCR | 0/0 | 2/2 | 3/2 | 2/3 | 1/0 | 0/0 | 0/0 |
Commercial monoclonal antibody-based sandwich ELISA | 0/0 | 0/0 | 0/0 | 2/1 | 0/0 | 0/0 | 0/0 |