Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action

Table 2 List of antibodies used in the experiments

Protein	Manufacturer, catalog no.	Dilution
RUNX-1	Santa Cruz Biotechnology, INC. sc-365644	1:100
RUNX-2	Santa Cruz Biotechnology, INC. sc-390351	1:100
Cathepsin K	Santa Cruz Biotechnology, INC. sc-48353	1:100
TRAP	Santa Cruz Biotechnology, INC. sc-376875	1:100
OPG	Santa Cruz Biotechnology, INC. sc-390518	1:50
RANKL	Santa Cruz Biotechnology, INC. sc-377079	1:100
OPN	Abcam, ab8448	1:1000
COL1-A1	Santa Cruz Biotechnology, INC. sc-293182	1:50
CASP-3	Invitrogen, 43-7800	1:250
Beta-actin	Sigma Aldrich, A5441	1:10000
Anti-mouse IgG, HRP conjugated	Sigma Aldrich, A3562	1:5000
Anti-rabbit IgG, HRP conjugated	Sigma Aldrich, A3687	1:5000

RUNX-1 Runt-related transcription factor 1, RUNX-2 runt-related transcription factor 2, TRAP tartrate-resistant acid phosphatase, OPG osteoprogerin, RANKL receptor activator for nuclear factor κ B ligand, OPN osteopontin, COL1-A1 collagen type I alpha 1 chain, CASP-3 caspase 3, HRP horseradish peroxidase

ISSN: 1477-3155