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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Fig. 3

Comparisons of affinity and high-life extension between fenobody and traditional nanobody. a SDS-PAGE analysis of the 5 traditional nanobodies against NDV expressed by bacterial system and purified with Ni–NTA-6FF Column. Lanes 1–5: NDV-Nb-2, -4, -24, -30 and -49. b Comparisons of the 5 fenobodies and traditional nanobodies binding the NDV with a capture ELISA. c Binding affinity analysis of NDV-fenobody-4 and NDV-Nb-4 to NDV particles by capture ELISA. d Concentrations of NDV-fenobody-4 and NDV-Nb-4 in the blood from the different time points of the injected BABL/c

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Journal of Nanobiotechnology

ISSN: 1477-3155

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