Fig. 4
Effect of erianin and E/DMSNs on mitochondrial membrane potential and mitochondrial signaling pathway. a Flow cytometry analysis of mitochondrial membrane potential in HaCaT cells after treatment with erianin and E/DMSNs for 24 h. b Quantitative analysis of the percentage of JC-1 monomers rate in (a). c The expressions of Bcl-2, Bax, cytochrome c, cleaved caspase-3, and cleaved PARP proteins after treatment with erianin and E/DMSNs for 24 h. d Quantitation of Bcl-2, Bax, cytochrome c, cleaved caspase-3, and cleaved PARP proteins normalized to β-actin in (c) by using Image J software. Values are represented as means ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001, significantly different compared with the erianin group. #p < 0.05, ###p < 0.005, ####p < 0.001, significantly different compared with the control group