Fig. 8

Spread of T lymphocytes and changes in CD4⁺ and CD8⁺ T lymphocytes in the spleens and peripheral blood. The CD4⁺ T Lymphocytes were separated from the spleen (a), CD4⁺ T Lymphocytes were separated from the blood (b), CD8⁺ T Lymphocytes were separated from the spleen (c) and CD4⁺ T Lymphocytes were separated from the blood (d). The CD4⁺ and CD8⁺ T Lymphocytes were separated from the spleen and peripheral blood at specified time points, and were sorted by flow cytometry with PBS as a control. Data showed mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 (determined by one-way ANOVA followed by a Tukey–Kramer post hoc test)