Fig. 5

M1 macrophages (a) or M2 macrophages (b) were incubated with control (0.1% DMSO), free BRP-187 (10 µM), NP without drug, BRP-187 (10 µM) encapsulated into NP, or positive control (staurosporin 3 µM) for 24 h. Then, the release of LDH was analyzed using the Promega Cytotox 96^® assay. Values are normalized to the DMSO control (100%) and given as percentage of cell viability; data are means + S.E.M., n = 3