Fig. 8
ATX-LPN partially rescue CDDP-induced hearing loss. a In vitro culture of Organ of Corti. CDDP induced a significant loss of hair cells (Myosin VII, red), especially in the high-frequency areas. b Survival outer hair cell numbers in 1 mm length at different locations from apical end of each group. *p < 0.05, **p < 0.01, ***p < 0.001 as compared with CDDP, #p < 0.05 as compared with ATX + CDDP. c–e Mean ABR threshold of mice treated with cisplatin, ATX 1 mg/ml, 5 μl. ATX-LPN + CDDP. *p < 0.05, **p < 0.01, ***p < 0.001 as compared with CDDP, #p < 0.05 as compared with ATX + CDDP. f Immunohistochemistry of cochlea in apex/middle/basal area of BM. The pretreatment of protective drugs (ATX/ATX-LPN) effectively reduced the expression of caspase-3 (red fluorescence) and rescued more OHCs (green fluorescence)