Fig. 4
The binding ability of PPi-Far-PMs to hydroxyapatite particles. PPi-Far-PMs and Far-PMs were labeled with FITC and incubated with the commercial synthetic hydroxyapatite (a, c) or with the biotechnological hydroxyapatite (b, d). The images show 30-min incubations with FITC, Far-PMs-FITC, PPi-Far-PMs or PPi-Far-PMs-FITC. Magnification, ×40. Binding kinetics (c, d) were assessed by assaying the bound Far using HPLC at different time points. All data are mean ± SD (n = 3)