Table 2 Primer sequences designed for quantitative real-time PCR analyses
Gene name | Primer sequence (5′→3′) | T. m. (oC) | Product size (bp) | |
|---|---|---|---|---|
Actin beta (ACTB) | F | GTCCACCTTCCAGCAGATGT | 60.1 | 169 |
R | ATAAAGCCATGCCAATCTCG | 60.1 | ||
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F | GCTAAGGCTGTGGGGAAAGT | 60.5 | 161 |
R | TCAGCAGCAGCCTTCACTAC | 59.4 | ||
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B) | F | GTTATTCGGTGTTCGCTGGT | 60.0 | 122 |
R | GTAGACCAGAGCGACCTTGG | 59.9 | ||
Fibroblast growth factor 2 (FGF2) | F | GGCACTGAAATGTGCAACAG | 60.3 | 151 |
R | TCCAGGTCCAGTTTTTGGTC | 59.9 | ||
Lactate dehydrogenase A (LDHA) | F | CATGCCCACAACAAGATCAG | 60.1 | 128 |
R | CCTTTCAGCTTGTCCTCCAC | 59.8 | ||
Proliferating cell nuclear antigen (PCNA) | F | TGCACGCATTTGTAGAGACC | 59.9 | 187 |
R | AGTCAGCTGGACTGGCTCAT | 60.0 | ||
Myogenic factor 5 (MYF5) | F | CCAGGAGCTCTTGAGGGAAC | 61.3 | 196 |
R | ACTCTGCTCCGTCGCGTA | 60.9 | ||
Myogenic differentiation 1 (MYOD1) | F | AGCTCTCGCAGGAGAAACAG | 59.9 | 160 |
R | CTGGAGGCAGTATGGGACAT | 60.0 | ||
Myogenin (MYOG) | F | GGCTGAAGAAGGTGAACGAA | 60.4 | 149 |
R | CTGCTGGTTGAGGCTGCT | 60.3 | ||
Paired box 3 (PAX3) | F | CCGTGCTAGATGGAGGAAGC | 61.9 | 157 |
R | AGACACGGCTTGCGGTATG | 61.9 | ||
Paired box 7 (PAX7) | F | CAGTAGAGACAGGCCAAGC | 59.2 | 134 |
R | GGAGTTGGGAAGGAGTAGGG | 59.9 | ||