Fig. 1
a Native-PAGE analysis of dextran-mediated conjugation of CAT with VEGF. Lanes 1, 2, and 3 contain dextran, VEGF and CAT as control, respectively; Lane 4, constitutes the conjugate of CAT–VEGF after incubation time. b Gel filtration chromatogram using a Sephadex G-200 column. Elution was performed with PB (pH 7.0) at a flow rate of 0.6 mL min−1, and the absorbance of each fraction (300 µL/fraction) was monitored at 280 nm. Inset plots are CAT activity of each peak, which was evaluated via CL-based CAT assay using H2O2-sensitive TGA-CdTe QDs. c Native-PAGE analysis of CAT–VEGF conjugate after purification. Lanes 1 and 3 contain VEGF and CAT as control, respectively; Lanes 2, 4, and 5 correspond to peak 3, 2, and 1, respectively. d Colorimetric ELISA results for VEGF detection of each peak (Inset: the corresponding photographs)