Fig. 3

a Flow cytometry images of HGC-27 cells treated with HMON@CuS/Gd for 1–4 h (left), and corresponding quantification of green mean fluorescence intensity of FITC inside cells (right). b CLSM images of HGC-27 cells treated with HMON@CuS/Gd for 1–4 h (left), and corresponding quantification of CLSM images using the green mean fluorescence intensity of FITC (right). Scale bar: 30 μm. c Cell apoptosis images of HGC-27 cells (left) and statistical graph of total apoptotic rates (right), after treated with or without near infrared (NIR) irradiation, in the presence of HMON@CuS/Gd nanoparticle. d EdU images of HGC-27 cells (left) and statistical graph of EdU-positive rates (right), after treated with or without near infrared (NIR) irradiation, in the presence of HMON@CuS/Gd nanoparticle. Scale bar: 100 μm. Data are shown as mean ± SD, n = 3. *** indicates P < 0.001