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Table 2 Key measures for the sandwich LFA

From: Duplex Shiny app quantification of the sepsis biomarkers C-reactive protein and interleukin-6 in a fast quantum dot labeled lateral flow assay

Sandwich assay 0–20 nM

Green channel

QD-525-anti-CRP

Red channel

QD-605-anti-IL-6

Image processing software

ImageJ

MultiFlow app

ImageJ

MultiFlow app

LOB

Negative

0.33

Negative

0.46

LOD

2.28

1.27

1.38

2.28

LOQ

11.12

5.52

9.49

7.33

R2 of linear fit

0.89

0.95

0.83

0.97

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Journal of Nanobiotechnology

ISSN: 1477-3155

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