Table 1 The primer sequences used for qRT-PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | TCCAGTATGACTCTACCCACG | CACGACATACTCAGCACCAG |
Acan | GACAAGGACGAGTTCCCTGG | CTCCGGGGATGTGGCATAAA |
Col2a1 | GTCCTACAATGTCAGGGCCA | ACCCCTCTCTCCCTTGTCAC |
Adamts5 | GCCAAATGGTGTGTCTGACC | CGCAAGAGCGAGAACACTGA |
COX2 | GATGACGAGCGACTGTTCCA | CAATGTTGAAGGTGTCCGGC |
Il6 | ACAAGTCCGGAGAGGAGACT | ACAGTGCATCATCGCTGTTC |
iNos | GGTGAGGGGACTGGACTTTTAG | TCTCCGTGGGGCTTGTAGTT |
Mmp1 | TGGACCTGAATATGGACTTGCT | GCTGGATGGGATTTGGGGAA |
Mmp3 | GGCTGTGTGCTCATCCTACC | TGGAAAGGTACTGAAGCCACC |
Mmp13 | GGACAAAGACTATCCCCGCC | GGCATGACTCTCACAATGCG |