|
Suspensiona
|
Average Rct valueb
|
Concentration by Rct (copies/ml)c
|
Recovery ratio (%)d
|
Relative error (%)e
|
|---|
|
PBS
|
15,833
|
10,915.0
|
109.2
|
± 2.4
|
|
L-ascorbic acid (1 mM)
|
15,244
|
10,987.2
|
109.9
|
± 3.8
|
|
Fe2+ (1 mM)
|
15,784
|
11,577.6
|
110.8
|
± 4.2
|
|
Mg2+ (1 mM)
|
15,484
|
9,187.9
|
91.9
|
± 3.8
|
|
Cu2+ (1 mM)
|
15,662
|
12,523.3
|
125.2
|
± 2.9
|
|
Zn2+ (1 mM)
|
15,422
|
8512.7
|
88.1
|
± 3.3
|
- aWSSV concentration in suspension is 104 copies/ml
- bAverage Rct value of WSSV detection (n = 3)
- cWSSV concentration was calculated using the calibration curve (Fig. 3b)
- dRecovery was defined as \(\frac{\left\{\mathrm{Concentration by Rct}-{10}^{4}\right\}\mathrm{ copies}/\mathrm{mL}}{{10}^{4} \mathrm{copies}/\mathrm{mL}}\times 100\)
- eRecovery error was defined as \( \sqrt {\frac{1}{{n - 1}}\sum\limits_{{k = 1}}^{n} {\left( {x_{i} - \mathop x\limits^{ - } } \right)} ^{2} } \), where \({x}_{i}\) and \(\stackrel{-}{x}\) denote Rct and average Rct values (n = 3), respectively
