State-of-the-art polymeric nanoparticles as promising therapeutic tools against human bacterial infections

Cano, Amanda; Ettcheto, Miren; Espina, Marta; López-Machado, Ana; Cajal, Yolanda; Rabanal, Francesc; Sánchez-López, Elena; Camins, Antonio; García, Maria Luisa; Souto, Eliana B.

doi:10.1186/s12951-020-00714-2

Journal of Nanobiotechnology

Table 2 Selected relevant pre-clinical assays based on novel drug-loaded polymeric nanoparticles for the treatment of bacterial pulmonary infections

From: State-of-the-art polymeric nanoparticles as promising therapeutic tools against human bacterial infections

Bacteria	Loaded molecule	Polymeric matrix	Surface modifications	Dose	Admin. route	In vitro/In vivo Model	Results	Ref
Mycobacterium tuberculosis	–	Chitosan	Tri-mannose	100 µg/m	Cell exposure	A549 cell line Hep G2 cell line	Both un-grafted and grafted PNPs are similarly internalized by macrophages They profoundly remodel the response of M. tuberculosis-infected macrophages mRNA sequencing shows nearly 900 genes to be differentially expressed due to tri-mannose grafting (which are enriched for pathways involved in cell metabolism)	Coya et al. [66]
Chlamydia psittaci	C. psittaci Ags	Chitosan	–	80 μL PNPs-Ags	I.m I.n	BALB/c mice	PNPs-Ags mediate stronger humoral and mucosal responses PNPs-Ags immunization remarkably reduces bacterial load and the degree of inflammation in the infected lungs Furthermore, PNPs-Ags vaccination inhibits C. psittaci disseminating to various organs in vivo	Li et al. [70]
Mycobacterium tuberculosis	H1 Ag	PLGA	–	0.5 mg/mL	Cell exposure	THP-1 cell line	H1-PNPs are efficiently internalized by the THP-1 human macrophages Immunized mice show significant increase in the production of total serum IgG, its isotypes and inflammatory cytokines levels, compared to H1 alone H1 NP–vaccinated mice display significant reductions in lung and spleen bacillary load, and prolonged survival	Malik et al. [67]
Mycobacterium tuberculosis	H1 Ag	PLGA	–	50 μg PNPs/mouse	I.p	C57BL/6 mice		Malik et al. [67]
Pseudomonas aeruginosa	PopB/PcrH	PLGA	–	20 µL PNPs / mouse	I.p	FVB/N mice	PNPs-immunized mice show 3–fourfold higher Th17 responses both in the lung and in the spleen compared to mice immunized with empty PNPs or PopB/PcrH alone PNPs-immunized mice show significantly lower bacterial counts in the lungs and improved survival	Schaefers et al. [64]
Pseudomonas aeruginosa	Tobramycin	Alginate / Chitosan	Dornase α DNase	250 μg/mL	Injection	Galleria mellonella	Survival rates of 90% after injection of PNPs A treatment with NPs prior to infection provides a longer antibiotic protection DNase functionalization leads to a DNA degradation and improved NPs penetration Tobramycin NPs both with and without DNase functionalisation, exhibits anti-pseudomonal effects	Deacon et al. [65]

A549 human alveolar lung cell line, AMPs antimicrobial peptides, C. psittaci Ags Chlamydia psittaci antigens, H1 Ag bivalent antigen of Mycobacterium tuberculosis Ag85B and ESAT6 proteins, Hep G2 Human hepatocytes cell line, PopB/PcrH P. aeruginosa antigens, THP-1 human monocytic cell line

Back to article page

ISSN: 1477-3155

Contact us

Submission enquiries: journalsubmissions@springernature.com