Fig. 2
ExosomeMIF prevented the cardiac injury process mediated by LncRNA–NEAT1 transfer. a Heat map of lncRNAs differentially expressed between exosome and exosomeMIF. b LncRNA–NEAT1 expression was validated by qRT-PCR in exosome and exosomeMIF. *P < 0.05 vs exosome in Student’s t-test, n = 3. c LncRNA–NEAT1 expression was validated by qRT-PCR in MSCs after LncRNA–NEAT1 silencing. *P < 0.05 vs siRNA–LncRNA–NEAT1 in repeated measures ANOVA, n = 3. d LncRNA–NEAT1 expression was validated by qRT-PCR in exosomes after silencing LncRNA–NEAT1 in MSCs. *P < 0.05 vs Control; ▲P < 0.05 vs MIF + siRNA−LncRNA−NEAT1 in repeated measures ANOVA, n = 3. e Representative images of echocardiography exhibiting the changes in cardiac function in each group. Echocardiographic analysis of EF (f) and FS (g). h, i p27 and p16 mRNA levels were analyzed using qRT-PCR. j Cardiomyocytes were isolated from control, Dox−, Dox + exosomeMIF−, Dox + exosomeMIF+siRNA−LncRNA−NEAT1-, and Dox + exosomeMIF+siRNA−LncRNA−NT-treated mice. k Percentage of β-gal-positive cells. l Representative images of SA-β-gal staining. Each column represents mean ± SD of three independent experiments. *P < 0.05 versus Control; ▲P < 0.05 versus Dox; ○P < 0.05 versus Dox+ exosomeMIF+siRNA−LncRNA−NEAT1 in one-way analysis of variance, n = 3