Fig. 3

Influence of medium composition on biofilm formation. Bacteria in PBS were injected in the center of the channel over the course of one hour. The chamber was subsequently perfused with TSB medium and M9 medium simultaneously during 66 h. a Micrographs of the biofilm formed after 36 h of incubation show the different rates of biofilm formation. b Overview of the biofilm formed after 66 h, the difference in biofilm thickness due to the different medium compositions can be clearly seen. c Biofilm growth was assessed by quantifying the increase of surface coverage with time. Each box-plot represents the distribution of surface coverage based on ten images for each medium composition every hour