Fig. 3
Treatment of mouse prostate cancer xenografts with PEI/antimiR complexes. a For the generation of tumor xenografts in athymic nude mice (Foxn1nu) 5 × 106 PC3 cells in 150 µL PBS were injected subcutaneously into both flanks of mice. The PEI/antimiR complexes injected intraperitoneally every 2–3 days at the time points indicated in the figure. Right half of the picture shows representative pictures of mice injected with scrambled RNA (control) or antimiRs. b Analysis of tumor lysates by Western blotting, for alterations in the expression levels of miR-375 target genes SEC23A and PHLPP1. Left: representative samples; right: quantitation of all Western blot samples