Fig. 5
Treatment of local melanoma xenografts with antimiR/PEI complexes in mice. a For the generation of melanoma xenografts, 5 × 106 LOX cells were injected subcutaneously into both flanks of athymic nude mice. The PEI/antimiR complexes were injected intraperitoneally every 2–3 days at the time points indicated in the figure. Tumor sizes were determined by measuring all three dimensions. b Representative pictures of mice. c Representative examples of immunofluorescence staining of melanoma xenografts with antibodies directed against the miR-150 target MDM4. The right panel shows the quantitation of immunofluorescence staining