Fig. 10

The effect of serum concentration on the cellular uptake of mTHPC-loaded nanovesicles in co-culture spheroids. a–c Cumulative mTHPC uptake in spheroids incubated in media supplemented with 2% ( ), 6% ( ) and 10% ( ) of FBS in the function of MeWo content. mTHPC uptake was measured as the mean fluorescence intensity of individual cells after 24 h incubation with NPs. d–f 2D contour plot of mTHPC uptake in the function of serum concentration and content of MeWo cells in spheroids. g Pearson correlation coefficients (PCC) between mTHPC uptake and serum concentration in culture medium ( ) or MeWo content in spheroid ( ). The concentration of mTHPC was 4.5 µM. * p < 0.05; ** p < 0.01 and *** p < 0.001