Fig. 1

Synaptosomes isolation and characterization. a Representation of synaptosomal particles with pre- and post-synaptic areas evidenced. b Synaptosomal band (red arrow) collected at sucrose interface after ultracentrifugation in sucrose fractionation. c Western blot of proteins extracted from total homogenate (Total) and synaptosomal fraction (Synapt) incubated with anti-PSD95 (PSD95) and anti- Synaptophysin (SyP). d Relative quantification of the immunoreactive bands. The uniformity of gel loading was confirmed by using β-actin as a standard. *P < 0.05, vs total homogenate (Total). e Intensity weighted size distribution functions of the synaptosomes extracted. f AFM images of the synaptosomes extracted