Fig. 4

Cytotoxicity assessment after different treatments. Cell viability of BV2 after incubation with different formulations of nanoparticles for 24 h (a), 48 h (c), 72 h (e) and the same operations on SHSH-SY5Y for 24 h (b), 48 h (d), 72 h (f). Data from CCK8 assay were expressed as mean ± standard deviation (n = 5). ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was used to assess the statistical differences between groups. Symbols represented statistical significance of the labeled groups with group of TMP.HCl (^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001)