Fig. 3

HuNb1-IgG4 induces phagocytosis and shows high activity of anti-tumor in human ovarian tumor-engrafted mice models. a Representative result for phagocytosis of CFSE-labeled Jurkat E6.1 cells phagocytosed by macrophages. The results were analyzed by FACS and showed in a bar graph. The experiment was performed in triplicate and data were presented as mean ± SD. b BALB/c nude mice were subcutaneously transplanted with SKOV3 cells and treated with low (0.2 mg/kg), medium (1 mg/kg) and high (5 mg/kg) dose of HuNb1-IgG4 and high (5 mg/kg) dose of Hu5F9-G4 or PBS as the control (n = 8). Tumor tissues from all mice in each group were shown. c Tumor volumes were measured and the average volume is shown. d Tumor weights were measured and shown in the graph. e H&E staining of SKOV3 tumors from control mice and mice treated with 5 mg/kg Hu5F9-G4 or different dose of HuNb1-IgG4. Magnification of ×400. The above animal experiments were performed in triplicate and one representative experiment was displayed. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus control