Fig. 3

Sizes and structural analysis of siRNA/ELPs complexes and intracellular trafficking. a TEM images of nanoparticle-like structures formed by siRNA/ELPs complexes at room temperature. Scale bar = 100 nm. b In order to determine subcellular localizations and siRNA release, MDA MB231 cells were stained with anti-EEA1 (early endosome antigen) after incubating them with siRNA/ELP complexes for 1 h at 37 °C. The cellular localizations of complexes in early endosomes were observed by confocal microscopy. Representative confocal microscopic images of five experiments. Blue, nuclei stained with Hoechst; Green, siRNA/ELP variant complexes; Red, Anti-EEA1. Scale bar, 10 µm