Fig. 3

Delivery efficiency and toxicity of TAP/PMOE50 complexes in a C2C12E50 cell line determined by fluorescence microscopy and flow cytometry. a Representative fluorescence images of PMOE50-induced exon-skipping in C2C12E50 cell line. (The images were taken 4-day after treatment. Original magnification ×200; scale bar 200 µm). b Statistical date of TE of PMOE50 formulated with TAP (Student’s t-test, *p ≤ 0.05 compared with PMO only). c Cell viability (Student’s t-test, *p ≤ 0.05 compared with untreated cell as control). In this test, 5 µg PMOE50 was formulated with TAPs (10 µg), and PEI 25 (5 µg), Endoporter (5 µg) formulated as comparison in 0.5 mL 10% FBS-DMEM medium, respectively. The results are presented as the mean ± SD in triplicate