Fig. 4From: Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike proteinDevelopment of multivalent nanobodies for highly efficient pseudovirus neutralization. a A schematic view of the multivalent nanobodies design. Homodimer, heterodimer and homotrimer were tandem linked with tail-to-head mode by flexibility linker. b The expression and purification of multivalent nanobodies fusions were analyzed with SDS-PAGE. M: Marker; lane 1 to 7: monoNb91-hFc, biNb91-hFc, triNb91-hFc, monoNb3-hFc, biNb3-hFc, triNb3-hFc and Nb91-Nb3-hFc, respectively. c and d Determination the binding affinity of multivalent nanobodies and monovalent Nbs with RBD protein using indirect ELISABack to article page