Fig. 5
From: Tailored liposomal nanotraps for the treatment of Streptococcal infections
Inhibition of secreted hemolysins of GAS and GGS supernatants or recombinant SLO by liposomes composed of synthetic PCs with saturated or monounsaturated acyl chains. PC-liposomes containing fully saturated acyl chains provide full protection against recombinant SLO; whereas liposomes containing a monounsaturated acyl chain at any position are unable to neutralize recombinant SLO (a). Liposomes composed of monounsaturated PC provide full protection against hemolytic activity of GGS 5804 supernatant, partial protection against GGS 5109, GGS ATCC 12394 supernatants and no protection against any GAS supernatants (b). Liposomes composed of saturated PC provide full protection against all GAS/GGS supernatants (c). Ch:Sm-liposomes or liposomes composed of monounsaturated PC provide only partial protection against hemolytic activity of GGS ATCCC 12394 supernatant; however, their combination is fully protective (d). Liposomes containing fully saturated acyl chains provide full protection against hemolytic activity of GGS ATCC 12394 supernatant; whereas liposomes containing a monounsaturated acyl chain at any position are only partially protective (e). The length of saturated acyl chains does not affect the liposomal neutralization of the hemolytic activity of GGS ATCC 12394 supernatant (f) or recombinant SLO (g). Error bars = Mean ± SD. N ≥ 3