Fig. 2
Effects of free drugs and NLCs on primary human neutrophils (6 × 105 cells/ml): a neutrophil cytotoxicity assay by LDH determination; b the measurement of extracellular superoxide production; c the uptake of rhodamine 800-loaded NLCs by human neutrophils observed via confocal microscopy; and d the fluorescence intensity of rhodamine 800 in the neutrophils analyzed by flow cytometry. All data are expressed as the mean ± SEM (n = 3). * p < 0.05 as compared to control group. LDH, lactate dehydrogenase; DAPI, 4’,6-diamidino-2-phenylindole