Fig. 5

Remodeling of the tumor immune microenvironment. Intratumoral levels of cytokines IFN-γ (a) and IL-2 (b) as determined by ELISA. c Representative images of Ki-67-stained (scale bar, 100 µm) and H&E-stained (scale bar, 100 µm) tumor tissue. d Representative immunofluorescence images of tumor slices stained with a hypoxia maker. e Quantitative analysis of the hypoxia marker (histogram). f Representative immunofluorescence images of tumor slices stained with CD86 (M1, red), CD206 (M2, green), and CD8 (red), respectively; the nucleus was stained with DAPI (blue). Corresponding quantitative analysis (histogram) of the M1 and M2 (g) and CD8 T cells (h)