Fig. 4

MIF-Exo effectively preserved cardiac function in rats with MI in vivo. a The flowchart of experimental design in vivo. The protein expression level of CD31 (b) and a-actin (c) was detected by immunofluorescence after Dil labeled exosomes taken by endothelial cells and cardiomyocytes 6 h after intramyocardial injection. (n = 3 biological replicates; 4 random fields for each animal). LVEF and LVFS were measured 2 and 4 weeks post MI (d), and quantification analysis (f). (2 weeks: n = 5 animals for each group; 4 weeks: n = 4 animals for MSC-Exo group, n = 5 animals for the other groups). Continuous variables and categorical variables were described by means ± SEM and percentages. *P < 0.05; ***P < 0.001; NS, not significant