Fig. 4

LbL coating maintained HFSC properties. a, b Flow cytometry was conducted on day 7 after culture. The results showed that the proportion of CD200⁺a6⁺ HFSCs at P0 or P2 in the coated group was higher than the uncoated group (mean ± SD; n = 3; **p < 0.01, Student’s t-test). c, d Immunofluorescence staining was carried out on day 7 after culture. The proportion of CD200⁺a6⁺ HFSCs at P0 or P2 in the coated group was higher (mean ± SD; n = 4; **p < 0.01, Student’s t-test). DAPI (blue); CD200 (red); a6 (green); scale bars: 20 µm. e The long-term cell culture under LbL coating results are from flow cytometry analysis of cells in each passage (mean ± SD; n = 3)