Fig. 1

In vivo biodistribution of hydrophobically modified glycol chitosan (HGC) nanomicelles. a Schematic representation of the preparation of HGC nanomicelles loaded with tacrolimus (HGC-TAC). b The FE-TEM image of HGC-TAC nanomicelles. c Fluorescence images of organs at different time intervals after injection of HGC-F675 in MRL/lpr mice. The near-infrared images of dissected organs were obtained using a near-infrared filter in a fluorescence-labeled organism bio-imaging instrument. The fluorescence intensity of organs was quantified at each time point (n = 3 mice/group). d Confocal images of HGC-F675 nanomicelles in the kidney at different time points after injection in MRL/lpr mice. Note that podocin is a marker of podocytes, indicating the glomerulus. Original magnification ×400 or ×200, respectively. Bar = 50 μm