Fig. 5
From: Sustained delivery of MMP-9 siRNA via thermosensitive hydrogel accelerates diabetic wound healing
In vitro transfection efficiency of PM1(GT/siRNA10) at Day-1, Day-4, Day-7. a Flow cytometry analysis of transfected HaCaT cells by released GT/siFAM10 from PM1 hydrogel. b Cellular uptake after treatments with released GT/siFAM10 from PM1 hydrogel. c siCy5 uptake of HaCaT cells after treatments with released GT/siCy510 from PM1 hydrogel by CLSM (scale bars = 50 μm). d Levels of MMP-9 mRNA in HaCaT cells by qRT-PCR and e Levels of MMP-9 protein in HaCaT cells by Western Blot after treatments with released GT/siMMP-910 from PM1 hydrogel. *P< 0.05 compared with negative control