Fig. 4

Influence of FRLnc1 knockdown on proliferation and migration of GC cells. a the influence of sh-FRLnc1 transfected on the expression of FRLnc1 in HGC-27 cells; b the growth of HGC-27 cells treated with sh-FRLnc1 transfected detected by cell growth curve; c the clone-forming ability of HGC-27 cells treated with sh-FRLnc1 transfected detected by cell clone-forming experiment; d the mRNA expression of Cyclin D1 in sh-FRLnc1 transfected HGC-27 cells detected by qRT-PCR; e the cell cycle of sh-FRLnc1 transfected HGC-27 cells detected by flow cytometry; f the cell apoptotic rates of sh-FRLnc1 transfected HGC-27 cells detected by flow cytometry; g the mRNA expressions of Bcl-2 and Bax in sh-FRLnc1 transfected HGC-27 cells detected by qRT-PCR; h the migration ability of sh-FRLnc1 transfected HGC-27 cells detected by Transwell migration test; i the mRNA expressions of EMT related genes in sh-FRLnc1 transfected HGC-27 cells detected by qRT-PCR; and j the protein expressions of cyclin D1, Bcl-2 and EMT related genes in sh-FRLnc1 transfected HGC-27 cells detected by Western blot, and the influence of exosomes containing FRLnc1 on phosphorylated ERK signaling pathways in HGC-27 cells