Fig. 3

Cell uptake of PTX@GO-PEG-OSA NSs (the concentration of PTX was 8 µg/mL). a Bio-TEM images of HGC-27/PTX cells, after incubation with DMEM plus 10% FBS (control) or PTX@GO-PEG-OSA NSs for 48 h. Yellow arrows represent PTX@GO-PEG-OSA NSs. b Inversed fluorescent microscopy images of HGC-27/PTX cells incubated with GO-PEG-OSA NSs for 1, 2, 3 and 4 h. Scale bar, 50 µm. c Concentration of PTX in the treated cells determined using UHPLC. d CLSM images (up) and fluorescence intensity correlation plot (down) of HGC-27/PTX cells incubated with GO-PEG-OSA NSs for 24 h. Scale bar, 500 nm. Data are shown as the mean ± SD, n = 3