Fig. 4
In vitro antitumor effect of PTX@GO-PEG-OSA NSs. a Viabilities of HGC-27/PTX cells after incubation with free PTX, PTX@GO-PEG-OSA and GO-PEG-OSA NSs, while the DMEM with 10% FBS was used as the negative control (blank) group. b EdU images and statistics EdU-positive number of HGC-27/PTX cells after the incubation with PTX, PTX@GO-PEG-OSA and GO-PEG-OSA NSs for 48 h. Scale bar, 100 µm. c Flow cytometry images and statistics apoptotic number of HGC-27/PTX cells after the incubation with PTX, PTX@GO-PEG-OSA and GO-PEG-OSA NSs for 48 h. d Expression of caspase-3, cleaved caspase-3, Bax, Bcl-2, p53 proteins and GAPDH protein in HGC-27/PTX cells after the incubation with PTX, PTX@GO-PEG-OSA and GO-PEG-OSA NSs for 48 h. e Viabilities of HGC-27/PTX cells, with or without treatment by NH4Cl, with the presence of PTX@GO-PEG-OSA NSs for 48 h. Data are shown as the mean ± SD, n = 3. ** indicates P < 0.01, *** indicates P < 0.001