Fig. 3

Cellular uptake of cPRP nanoparticles by HeLa cells. The cells after 1 and 3 h of incubation with the DiD@cPRP nanoparticles with or without cRGD pretreatment were monitored by a CLSM, b flow cytometry profiles, and mean fluorescence intensity. The red fluorescence indicates the DiD@cPRP nanoparticles, and the blue fluorescence indicates the cell nuclei. c DiD delivery tracking in HeLa cells after 2 and 4 h of incubation with free DiD and DiD@cPRP nanoparticles, as detected with CLSM. The blue, green, and red fluorescence indicate cell nuclei, lyososomes and DiD, and DiD@cPRP nanoparticles, respectively. Scale bar: 25 μm. Data are shown as mean ± SD (n = 3) (**P < 0.01)