Fig. 2
a SDS-PAGE of samples collected during ZHer2 preparation. M, protein marker; lane 1, total protein of uninduced cells; lane 2, total protein of induced cells; lane 3, soluble protein of induced cells; lane 4, insoluble protein of induced cells; lane 5, flow-through fractions after binding to Ni–NTA resin; lane 6, protein purified by Ni–NTA affinity chromatography. b SDS-PAGE of the purified ZHer2 affibody in the presence or absence of 2-ME. c SDS-PAGE characterization of ZHer2 labelled with 6-FAM. M, protein marker; lane 1, unlabelled ZHer2; lane 2, 6-FAM-labelled ZHer2. d HAADF-STEM images of Pt@mPDA/MnO2/PDA-ZHer2 NPs. Element mapping of Pt@mPDA/MnO2/PDA-ZHer2 NPs: e merged image, f C, g N, h O, i S, j Mn, and k Pt