Table 1 List of the D1 site directed mutants selected for the systematic characterisation of potential biorecognition element
Site directed mutants | WT | Mutant | Hydropathic index/reactivity class/side chain polarity | Localization of the mutation in the protein D1 | |||
|---|---|---|---|---|---|---|---|
WT | Mutant | ||||||
A153S | Alanine | Serine | 1.8 (II) | Nonpolar | − 0.8 (0) | Polar | In the helix iii of d1 |
A250L | Alanine | Leucine | 1.8 (II) | Nonpolar | 3.8 (IV) | Nonpolar | In the stroma loop ii |
A250R | Alanine | Arginine | 1.8 (II) | Nonpolar | − 4.5 (0) | Polar | In the stroma loop ii |
A250V | Alanine | Valine | 1.8 (II) | Nonpolar | 4.2 (III) | Nonpolar | In the stroma loop ii |
A251C | Alanine | Cysteine | 1.8 (II) | Nonpolar | 2.5 (V) | Polar | In the stroma loop ii |
F182M/I184M | Phenylalanine | Methionine | 2.8 (VI) | Nonpolar | 1.9 (V) | Nonpolar | In the helix v of d1 |
Isoleucine | Methionine | 4.5 (IV) | Nonpolar | 1.9 (V) | Nonpolar | ||
F255N | Phenylalanine | Asparagine | 2.8 (VI) | Nonpolar | − 3.5 (0) | Polar | In the stroma loop ii |
F265S | Phenylalanine | Serine | 2.8 (VI) | Nonpolar | − 0.8 (0) | Polar | In the ii stroma loop |
F265T | Phenylalanine | Threonine | 2.8 (VI) | Nonpolar | − 0.7 (0) | Polar | In the ii stroma loop |
G207S | Glycine | Serine | − 0.4 (I) | Nonpolar | − 0.8 (0) | Polar | In the helix iv of d1 |
L159I | Leucine | Isoleucine | 3.8 (IV) | Nonpolar | 4.5 (IV) | Nonpolar | Near to tyr 161 |
L159V | Leucine | Valine | 3.8 (IV) | Nonpolar | 4.2 (III) | Nonpolar | Near to tyr 161 |
L159M | Leucine | Methionine | 3.8 (IV) | Nonpolar | 1.9 (V) | Nonpolar | Near to tyr 161 |
L200I | Leucine | Isoleucine | 3.8 (IV) | Nonpolar | 4.5 (IV) | Nonpolar | In the helix iv of d1 |
I163F | Isoleucine | Phenylalanine | 4.5 (IV) | Nonpolar | 2.8 (VI) | Nonpolar | Near to tyr 161 |
I163N | Isoleucine | Asparagine | 4.5 (IV) | Nonpolar | − 3.5 (0) | Polar | Near to tyr 161 |
I163S | Isoleucine | Serine | 4.5 (IV) | Nonpolar | − 0.8 (0) | Polar | Near to tyr 161 |
I163T | Isoleucine | Threonine | 4.5 (IV) | Nonpolar | − 0.7 (0) | Polar | Near to tyr 161 |
I281T | Isoleucine | Threonine | 4.5 (IV) | Nonpolar | − 0.7 (0) | Polar | In the helix v of d1 |
M172L | Methionine | Leucine | 1.9 (V) | Nonpolar | 3.8 (IV) | Nonpolar | Near to oec |
P162S | Proline | Serine | − 1.6 (III) | Nonpolar | − 0.8 (0) | Polar | Near to tyr 161 |
S212C | Serine | Cysteine | − 0.8 (0) | Polar | 2.5 (V) | Polar | In the helix iv of d1 |
S264K | Serine | Lysine | − 0.8 (0) | Polar | − 3.9 (0) | Polar | In the stroma loop ii |
S268C | Serine | Cysteine | − 0.8 (0) | Polar | 2.5 (V) | Polar | In the stroma loop ii |
S209A/S212C | Serine | Alanine | − 0.8 (0) | Polar | 1.8 (II) | Nonpolar | In the helix iv of d1 |
Serine | Cysteine | − 0.8 (0) | Polar | 2.5 (V) | Polar | ||