Fig. 3

Cellular uptake and lysosomal escape of DA-P-SS-T/PTX nanomicelles. A CLSM images of A549 cells pretreated with chlorpromazine, genistein, and amiloride and then treated with DA-P-SS-T/C6 nanomicelles. The nuclei were stained by DAPI (blue). *P < 0.05, vs. PBS. B CLSM images of lysosomal escape of nanomicelles in A549 and A549/ADR cells. Green fluorescence signal corresponds to C6. The late endosomes and lysosomes were labeled by LysoTracker Red. Yellow fluorescence signal corresponds to overlay of the C6 and lysosomal signals. Scale bars are 20 μm. (1) CLSM; (2) The intensity of the fluorescence signal in the corresponding images or Pearson coefficient and Mander coefficient. *P < 0.05, **P < 0.01 vs. DA-P-SS-T/PTX