Fig. 4

In vitro mitochondria-targeting delivery and assay of the mitochondrial membrane potential. A Tracking of C6 delivery in A549 and A549/ADR cells based on the MitoTracker signal corresponding to the mitochondria (red) and C6 signal (green) by CLSM. *P < 0.05, **P < 0.01 vs. DA-P-SS-T/C6. B Colocalization analysis. C A549 and A549/ADR cells treated with DA-P-SS-T/C6 were labeled with JC-1 to detect mitochondrial depolarization by CLSM and compare the signal with that in the control cells. Green and red colors correspond to the signal of monomeric and aggregated forms of JC-1, respectively. (1) CLSM image; (2) Pearson coefficient and Mander coefficient or the JC-1 monomer/JC-1 aggregate ratio