Fig. 5

Internalization of cationic carbosilane dendrimers in PBMCs. Confocal microscopy images of PBMCs treated for 1, 2 or 6 h with 5 µM G2-SN15-PEG FITC-dendrimer and 1 µM G3-SN31-PEG FITC-dendrimer (a). PBMCs were fixed and stained with Phalloidin (red, actin filaments) and DAPI (blue, nucleus). Confocal images of random field (b). Entry of FITC labeled dendrimers into PBMCs observed by flow cytometry at 1, 2 and 6 h post-treatment. DAPI 4′,6-Diamidino-2-phenylindole dihydrochloride