Fig. 6

Internalization of cationic dendrimers in U87MG-CD4⁺CCR5⁺ cells. Confocal microscopy images of U87MG-CD4⁺CCR5⁺ cells treated for 1, 2 or 6 h with G2-SN15-PEG FITC 1 µM and G3-SN31-PEG FITC 0.5 µM (green) FITC-labeled dendrimers. Cells were fixed and stained with Phalloidin (red, actin filaments) and DAPI (blue, nucleus). a Confocal images of random field. b Entry of FITC labeled dendrimers into PBMCs observed by flow cytometry at 1, 2 and 6 h post-treatment. DAPI 4′,6-Diamidino-2-phenylindole dihydrochloride